In summary, m7G-quant-seq could be broadly applied toquantitatively monitor tRNA m7G methylation level changein diverse biological processes, such as gene knockdown, cellularstress, heat shock, cancer progression, etc. Considering the METTL1-mediatedtRNA m7G regulates tumorigenesis and cancer progressionin many cancer types, m7G-quant-seq could potentially facilitatea series of future investigations on METTL1 functions in cancer biologyand cancer therapy. This evidence concerns the gene METTL1 and cancer.