To further explore the effects of SH3BP5-AS1 on the biological behavior of PC cells, PC cells (BxPC3, MIA Paca-2, and PANC-1) were transfected with lentiviral vectors encoding SH3BP5-AS1 inserts or short hairpin RNA (shRNA) and Transwell and sphere formation assays were conducted. The gene discussed is SH3BP5; the disease is pachyonychia congenita.