Furthermore, we showed through western blotting, RT-qPCR and IHC techniques that EDBRB loss or inhibition resulted in obvious reduced levels of EDNRB, GRK2, NF-κB, IKKα, IKKβ, p-NF-κB, p-IKKα and p-IKKβ in liver tissues of PBC mice (Fig. 7B–H). The gene discussed is CHUK; the disease is primary biliary cholangitis.