DLGAP4 and hepatocellular carcinoma: In addition, we used qRT‒PCR and Western blotting to detect the expression of DLGAP4 mRNA and protein in HCC cell lines (MHCC97h, HCCLM3, HepG2) and normal liver cells (LO2), and the results showed that the expression of DLGAP4 in HCC cell lines was significantly higher than that in normal liver cells (P < 0.05) (Fig. 6C,D).