This was associated with increased intracellular oxidative stress, indicated by elevated reactive oxygen species (ROS) and inducible nitric oxide synthase (iNOS) levels (Figure 1F), as well as induction of the proinflammatory cytokines IL-6, IL-1β, and TNF-α (Figure 1G) and TGF-β (Figure 1H) in kidney macrophages of CKD mice compared with controls. This evidence concerns the gene NOS2 and chronic kidney disease.