To discover the function of the anti-NanAT1-TufT1-PlyD4 specific antisera based on their surface recognition of S. pneumoniae, cell adhesion assays were carried out in vitro, and the results demonstrated that anti-NanAT1-TufT1-PlyD4 antisera inhibited the adhesion of pneumococcus to A549 cells; these results supported that the protection was enhanced through humoral immunity against invasive pneumococcal infection, which was elicited by immunization with NanAT1-TufT1-PlyD4. Here, TUFT1 is linked to pneumococcal infection.