In the study of liver injury, rhein has been found to help alleviate liver fibrosis caused by CCL4 (11), and for chronic liver injury caused by methotrexate (12) (MTX), rhein has been shown to inhibit the elevation and remission of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) through the Nrf2-HO-1 pathway. Here, GPT is linked to Hepatic fibrosis.