The results revealed that bile Wisteria floribunda Agglutinin (WFA)-Positive Mucin 1 is a promising CCA biomarker, which can effectively distinguish CCA from benign biliary tract diseases with sensitivity and specificity of 90.0% and 76.3%, respectively and the AUC was 0.86 (51). Here, MUC1 is linked to cholangiocarcinoma.