The relationship between NRGPI and cancer progression pathways, such as WNT and TGF-β signaling pathways, was also confirmed as the mRNA expression level of WNT and TGF-β markers (wnt markers: WNT2B, WNT9A; TGF-β markers: TGFB1 and TGFB3), which were selected based on correlation analysis (Supplementary Figures 5C, D), were also significantly elevated with induction of necroptosis. This evidence concerns the gene TGFB1 and cancer.