GLS and neoplasm: As shown in Figure 5H, after cryo-thermal therapy, the expression level of glutaminase, the key enzyme responsible for the transformation of glutamine to glutamate (23), was significantly upregulated compared with that in the tumor-bearing control, but the expression level of glutaminase was downregulated after combination therapy compared to cryo-thermal therapy alone, which suggested that glutamine metabolism was significantly decreased by combination therapy.