Other small macrophage subpopulations that clustered separately were identified as Cd248 M2-like, marked by the expression of Cd248, Itgae resident macrophages, marked by the expression of both Cd74 and Itgae, and proliferative macrophages, marked by the expression of Mki67. These findings indicate the complexity of macrophage subpopulations in both dystrophic and severely dystrophic environments and the involvement of dynamic macrophage phenotypes in the pathological process of muscular dystrophy (Singh and Chazaud, 2021). This evidence concerns the gene CD248 and muscular dystrophy.