TLR4 and prion disease: At level 3 of KEGG, pathways from two groups with significant statistical differences ranked from top to the bottom in bar graphs based on the Wilcoxon method, suggesting that exposure to microplastics may lead to differences in Toll and Imd signaling pathways, Autophagy-yeast, and Cardiac muscle contraction in nasal microbiota (Figure 4E), and differences in cardiac muscle degradation, Prion diseases, Staphylococcus aureus infection, styrene degradation, biosynthesis of unsaturated fatty acids, and nitrotoluene in intestinal microbiota (Figure 4F).