Folic acid supplementation can regulate the transcription of NADPH oxidase, effectively reduce the oxidative stress caused by high-fat diet (40), increase AMP levels, and activate LKB1 to restore AMPK activation in the liver, thereby improving cholesterol and glucose metabolism, which may slow down the pathogenesis of NAFLD (41). The gene discussed is FMO5; the disease is metabolic dysfunction-associated steatotic liver disease.