In addition, tRF-Glu-TTC-047 was shown to be a highly expressed tsRNA in IUGR porcine skeletal muscle which might be involved in skeletal muscle development by targeting IGF1. Our results provide a reference for the study of tsRNAs in skeletal muscle and offer new insights into understanding the impacts of IUGR on skeletal muscle in animals. Here, IGF1 is linked to fetal growth restriction.