Supplementary Figure S4 shows the importance of 10 genes in the RF model. The forecast performance of each gene in both NAFLD and DN was shown in Supplementary Figure S5. The area under the curve (AUC) values of LPL and SPP1 in DN were 86% and 80.1%, and the AUC values of LPL and SPP1 in NAFLD were 72.5% and 64.3%, respectively. Supplementary Figure S6 showed the expression of the 10 genes in the two diseases. The gene discussed is SPP1; the disease is metabolic dysfunction-associated steatotic liver disease.