Ultimately, the expression of the 10 optimal crosstalk genes in NAFLD [CD36, WIPI1, CBX7, FCN1, SLC35D2, CP, ZDHHC3, PTPN3, lipoprotein lipase (LPL), and SPP1] was input to create the RF model. This evidence concerns the gene LPL and metabolic dysfunction-associated steatotic liver disease.