To study the ability of EVOO extract to modulate the expression of circulating mononuclear cells involved in chronic diseases such as obesity, the expression of CD14+CD16+ cell population was evaluated by cytofluorimetric analysis among the monocyte population (CD64+CD45+ cells; gating strategy is reported in Supplementary Figure 1). This evidence concerns the gene CD14 and obesity due to melanocortin 4 receptor deficiency.