In the current study, we found that paternal n-3 PUFA supplementation in preconception reduced anxiety- and depressive-like behavior (OFT, LDT, SPT), improved sociability (TCT), learning and memory (NOR) in the offspring, along with increased synaptic number, upregulated expressions of NSE, GFAP, MBP, BDNF in the hippocampus and cerebral cortex, as well as altered expressions of genes associated with mitochondria biogenesis, fusion, fission and autophagy. This evidence concerns the gene MBP and Anxiety.