In addition, we used qRT-PCR to compare the expression of hub genes (CHMP4C, GSDMB, NOD2, PLCG1, CYCS, GPX4) between prostate cancer cell lines (LNCap, PC3, DU-145) and the normal prostate epithelial cell line (RWPE-1). The gene discussed is PLCG1; the disease is Familial prostate cancer.