The correlation between the expression of signature genes and the prognosis of patients with NSCLC was evaluated using KM-plotter and GEIPA database, and it was found that high expression of RNPC3 and low expression of RHEBL1 significantly prolonged the survival cycle of patients, and the key genes were analyzed for immune infiltration. The gene discussed is RNPC3; the disease is non-small cell lung carcinoma.