The introduction of the validation set GSE44077 to validate the five genes screened (Figure 4D) revealed that the expression levels of RNPC3 (p = 0.00036) in lung cancer tissues of non-small cell lung cancer were significantly higher than those of matched normal controls, and the expression levels of RHEBL1 (p = 6e-0.9) were significantly lower than those of matched controls; while DOCK6 (p = 0.81), PIWIL2 (0.24) and GPC (0.055)were not significantly different between the disease and normal control groups. The gene discussed is RNPC3; the disease is non-small cell lung carcinoma.