The results of our in vitro experiments validated that the mRNA expression of CALR was higher in BCA cells T24 than in normal urothelial cells SV-HUC-1 (Figure 9B); upon knocking down the gene expression of CALR in T24 cells, the viability of tumor cells reduced significantly (Figure 9D), a phenomenon consistent with the clinical outcomes. This evidence concerns the gene CALR and neoplasm.