Through GO and KEGG analysis, combined with PCR validation, it was further screened that G0S2, VEGFA and NUPR1 might participate in the apoptosis process of HK-2 cells induced by PPM1K. This study provides some data support for the study of the mechanism of HK-2 cell apoptosis, and also provides a scientific theoretical basis for the further study of the impact of PPM1K on kidney disease. This evidence concerns the gene NUPR1 and kidney disorder.