To better understand the therapeutic mechanism of HSSD in H1N1-infected mice, we measured the expressions of TLR4/NF-κB p65 and HIF-1α/IL17 pathways according to the prediction of network pharmacology results in lung tissues on the day 5 post-infection by using RT-qPCR and IHC staining. Here, NFKB1 is linked to infection.