As shown in Figures 7B,C, compared to CHv infection, UL47 density in both the nuclei and the cytoplasm was lower in ΔUS3 infection according to the mean value, indicating that US3 deletion reduced UL47 protein level, which was consistent with our results of UL47 expression in western blot assays (Figures 1A,B, 2A, 6). This evidence concerns the gene RPS3 and infection.