Western blot results revealed that the abundance of activated AKT was significantly increased and the expression of HIF-1α was also significantly up-regulated (Figures 5(a) and 5(b)) after overexpression of WDR72 in A549 stem cells, suggesting that the AKT/HIF-1α pathway is involved in WDR72 regulating lung cancer stem cells. This evidence concerns the gene HIF1A and lung cancer.