As a result, the SMAD4 protein expression level was relatively higher in the MIA-PaCa-2 model than in other PDAC cancer cell line models (Supplemental figure S1(a)), and PAI-1 protein increased in the 3 mg/kg group at 72 h after h4#147D administration (Figure 3(l)). The gene discussed is SMAD4; the disease is cancer.