Apart from more electrophysiology and fluorescent imaging techniques that require specialized equipment, gene and protein expression analysis, as well as inflammatory cytokine or neuropeptide release are commonly used in the investigation of itch mechanisms, e.g., measuring mediator release by enzyme-linked immunosorbent assays (ELISA, e.g., substance P, TSLP, NGF or histamine). The gene discussed is TSLP; the disease is Pruritus.