In the anxiety-like behavioral domain, Chd8+/S62X pups show largely normal mother separation-induced USVs, while Chd8+/S62X juveniles show partially increased anxiety-like behaviors (increased mother seeking but normal open-field center time [males]), which is followed by increased anxiety-like behavior in adults (decreased open-field center time [females] and increased closed-arm time [males and females]. Here, CHD8 is linked to Anxiety.