The results showed that the expression levels of ISCA1, ISCA2, C1ORF69 and NFU1 were significantly different in normal tissues versus KIRC tumor tissues (Fig. 3a, p < 0.01), among different tumor grades (Fig. 3b, p < 0.01), and among different individual cancer stages (Fig. 3c, p < 0.01). The gene discussed is ISCA1; the disease is neoplasm.