Similar to previous studies [26–28], the children with overweight/obesity presented an increased percentage of liver fat and total body fat as assessed by magnetic resonance imaging (MRI) as well as obesity-related alterations in metabolic and inflammatory serum parameters such as triglycerides, cholesterol, high-density lipoprotein and low-density lipoprotein (LDL), high sensitive c-reactive protein, alanine aminotransferase (ALAT), adiponectin, leptin, insulin, as well as Homeostatic Model Assessment-Insulin Resistance (HOMA-IR) (S1 Table). Here, INS is linked to obesity due to melanocortin 4 receptor deficiency.