Compellingly, ENCODE’s 450K-methylation array data for CRC cell lines HCT116 and Caco2 and our COBRA data for HCT116, Caco2, RKO, and SW480 clearly demonstrate that the proximal promoter region of SPRY4 is also unmethylated in CRC cells and therefore rule out this locus as potential contributor in regulating SPRY4 gene expression. This evidence concerns the gene SPRY4 and colorectal carcinoma.