USP1 is a member of the deubiquitinating enzyme family that can bind USP1‐associated factor to form a protein complex and participate in the regulation of DNA damage responses, mainly the FA pathway and TLS repair.[37, 38] Our study found that SERPINB3 in HNSCC cells could undergo cytonuclear translocation and colocalize with USP1 after treatment with cisplatin. This evidence concerns the gene SERPINB3 and head and neck squamous cell carcinoma.