In these models, this “immune exclusion” in the tumor microenvironment is mediated by upregulating signaling of the TGFβ pathway, and dual targeting of PD-L1 and TGFβ increased CD4+ and CD8+ T-cell infiltrates within the tumor microenvironment, which was not seen with inhibition of single targets alone (25). This evidence concerns the gene CD8A and neoplasm.