To investigate the role of GLS in tumor vasculature, we crossed C57BL/6 mice harboring floxed GLS alleles (GLSfl/fl, referred to as WT) with C57BL/6 mice expressing tamoxifen-inducible Cre recombinase (CreER) under the control of the Cdh5/vascular endothelial-cadherin gene promoter. Here, CDH5 is linked to neoplasm.