IRF3 and neoplasm: In the case of danger-associated stimuli, and since the expression of CGAS (that codes for cGAMP synthase) was higher in MTX-M-MØ compared to untreated M-MØ (Fig. 3a), we explored the effect of MTX on the cGAS-cGAMP-STING pathway, which detects tumor-derived DNA and generates antitumor immunity via the TBK1-IRF3-dependent production of IFNβ and the NFκB pathway [34].