Compared to untreated M-MØ, cGAMP induced higher STING activation (Fig. 3b) and higher levels of IL-6 and IFNβ1 in MTX-M-MØ (Fig. 3c), thus indicating that MTX also alters macrophages-responses to a danger-associated stimulus commonly present in the tumor microenvironment. This evidence concerns the gene IL6 and neoplasm.