EGF and cancer: While five of the cell lines behaved the same way as MCF10A cells and the primary cancer cells from the sixteen primary breast tumours studied, their aerotaxis depending on the EGF activation of EGFR, three cell lines strongly expressing EGFR seemed to performed aerotaxis independently of EGF binding, and two cell lines seemed to only depend accessorily on the EGF activation of EGFR.