In this study, we determined the levels of serum CTRP9 and different APN multimers in patients of different stroke stages and non-stroke subjects, to define the association of CTRP9/APN multimer levels, as well as dynamic changes after onset, with ischemic stroke, therefore to explore their potentials as risk factors and/or biomarkers. Here, C1QTNF9 is linked to stroke disorder.