To determine if oxidative stress similarly impacted cellular antioxidant responses in an acute cholestasis model, the expression in 3-day BDL mouse liver of the Nrf2 regulatory and response proteins Kelch-like ECH-associated protein 1 (Keap1), GCLC, HO-1, GSTμ and carbonyl reductase 3 (Cbr3) was examined and quantified by Western blot analysis (Fig 7A). Here, GCLC is linked to cholestasis.