NLRP3 and Hyperglycemia: In addition, some experiments have found that hyperglycemia (30 mM glucose for 48 h) induces the activation of NADPH oxidase through TRPM2 channel-mediated Ca2+ influx in monocytes, which contributes to ROS generation and thioredoxin-interacting protein -mediated activation of the NLRP3 inflammasome.