The results showed that the mRNA expression levels of the tested DPV genes in DEFs were significantly lower at the indicated time points after US1 deletion than after CHv deletion, but there was no difference in viral gene expression between 2ΔUS1R and CHv (Fig. 4), indicating that the US1 gene can promote the transcription of representative DPV IE (ICP4 and ICP27), E (TK), and L (gI, gC, and VP16) genes during viral infection (Fig. 4). The gene discussed is TKT; the disease is viral infectious disease.