The ability of colon cancer cells in proliferation, invasion and migration were evaluated, and BGN protein knockdown significantly reduced HT29 cells proliferation (Figure 6B), clone formation numbers in vitro (Figure 6C), migration and invasion (Figure 6D) as measured by the transwell assays, while THBS2 knockdown has little influence on colon cancer cells proliferation or colony formation abilities and only significantly reduced HCT116 migration and invasion ability. The gene discussed is THBS2; the disease is malignant colon neoplasm.