BGN and THBS2 knockdown significantly reduced colon cancer cells migration and invasion, as well as down‐regulating three EMT‐related proteins (Snail, Vimentin and N‐cadherin), and increasing the proliferation inhibitory effect of 5‐fluorouracil, irinotecan and oxaliplatin treatment. This evidence concerns the gene SNAI1 and malignant colon neoplasm.