As a gauge of neurodegeneration, we measured neurofilament light (NFL) in the same tissues by western blotting (Fig. 1c) and ELISA (Additional file 1: Fig. S1), and found that it was unaltered in the synucleinopathies compared controls, suggesting that the neuronal integrity of these tissues was still intact. This evidence concerns the gene NEFL and synucleinopathy.