LINC01194 and neoplasm: The suspended splenocytes were then removed by passage through a cell strainer which retained the 4T1 CSCsp and gene expression in the CSCsp was analyzed by RNAseq. In this experiment, 4T1 CSCsp were cultured alone or with splenic T cells primed in vivo by tumor antigen but with no ex vivo reinforcement of activation, or with the reinforced ex vivo activation of tumor antigen primed splenocytes.