Although no significant correlation was found between the frequency of CD4+IFN-γ+TNF-α+ T cells and disease parameters, the presence of IFN-γ monofunctional T cells (CD4+IFN-γ+TNF-α− T cells) in TDLNs of HNSCC had the most associations with poor prognostic indicators. Here, CD4 is linked to head and neck squamous cell carcinoma.