The frequencies of CD4+IL-4+, CD4+IL-17+ and CD8+IL-10+ showed significant increases in laryngeal SCC patients with tumor size ≤ 3 cm (P = 0.024, P = 0.048, P = 0.001, respectively) (Additional file 1: Fig. S11 and S12). This evidence concerns the gene CD4 and neoplasm.