In addition, in patients with tongue SCC, the frequency of CD4+CD25+Foxp3+CD127low/− cells was significantly lower in TDLNs of LN+ patients (patients with at least one involved LN) in comparison with LN– ones (patients without involved LN, P = 0.036, Additional file 1: Fig. S4). The gene discussed is CD4; the disease is tongue squamous cell carcinoma.