CASP1 and metabolic dysfunction-associated steatohepatitis: In addition, NLRP3 knock-in mice showed severe liver inflammation, with increased infiltration of activated macrophages in a diet-induced NASH model19, while NLRP3-/- mice and CASP1-/- mice showed decreased histological hepatic injury and serum ALT and AST levels when exposed to liver insults23,24.