To determine if S6K is involved in mediating the effects of chronic hyperglycaemia, we inhibited S6K with 10 μM PF-470867142, which reduced ribosomal S6 phosphorylation by ~50% and restored AMPK phosphorylation in HG-cells to control levels (Fig. 7a–c). This evidence concerns the gene RPS6KB1 and Hyperglycemia.