Hyperglycaemia-induced upregulation of several glycolytic genes was reduced by S6K inhibition, including aldolase B, Pdk1 and 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (Pfkfb3), a bifunctional enzyme that converts F6P to F2,6BP, a potent activator of phosphofructokinase. Here, PDK1 is linked to Hyperglycemia.