The results showed that the K63-mediated ubiquitination of ULK1 was significantly inhibited after knocking down endogenous ubiquitin (Fig. 6e); while K63R-mutant ubiquitin significantly blocked the AQP5 overexpression-induced self-renewal and migration of GC cells (Figure S10a, b). The gene discussed is AQP5; the disease is gastric cancer.