RSL3 treatment induced increased expression of ferroptosis marker TfR1 in several thyroid cancer cell lines compared to control (Figs. 2B, 5B, Supplemental Fig. 2A and 2B) despite varied basal levels (Fig. 1E), and significant rise in intracellular ROS and lipid peroxidation in thyroid cancer cells in vitro. Thus, we have demonstrated that RSL3 induces ferroptosis in thyroid cancer cells in vitro. This evidence concerns the gene TFRC and thyroid cancer.