As shown in Fig. 3A and B, the area of cell clone in RAB27A knockdown SW480 cells was significantly smaller than that in the negative control cells, whereas cell colon area in RKO cells with stable Flag-tag RAB27A expression was significantly larger than that in the negative control group (Fig. 3C and D), indicating that RAB27A is of vital importance for clone formation of colon cancer cells. Here, RAB27A is linked to colonic neoplasm.