Then, MTT and transwell assay were performed and the results indicated that the proliferation rate, as well as the migration and invasion ability, were higher in SW480 cells than that in RKO cells, which reveals that the expression level of RAB27A may positively correlate with the proliferation, migration and invasion ability of CRC cells (Fig. S3). The gene discussed is RAB27A; the disease is colorectal carcinoma.