To study the interactions between NK and TNBC cells, we captured single NK-92MI cells (interleukin-2 independent Natural Killer cell line), single MDA-MB-231 cells, and cancer-immune doublets (CIDs, one NK-92MI cell and one MDA-MB-231) and incubated the cells for 13 h using the Fluidigm Polaris system26,29–32 (Figs. 1a, S1). This evidence concerns the gene IL2 and cancer.