The results showed that RI-962 reduced the levels of pRIPK1, pRIPK3, and pMLKL proteins in the colon during DSS challenge, but did not impact the expression of RIPK1, RIPK3, and MLKL proteins (Fig. 9j), suggesting that RI-962 suppressed the RIPK1 signaling in the mouse model of DSS-induced colitis. This evidence concerns the gene MLKL and colitis.